Abstract
To develop a natural mineral-based electrochemical enzyme biosensor, natural molybdenite (MLN), tyrosinase (TYR), and acridine orange (AO) were coadsorbed onto a glassy carbon electrode (GCE). The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1-80 μM; sensitivity, 0.0315 μA/μM; LOD, 0.029 μM; response time, <4 >s) with good reproducibility and good operational and storage stabilities. The electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance with dissipation (QCM-D) revealed interesting roles of AO: (1) an efficient glue for enhancing the amount of the adsorbed TYR on the MLN-GCE, (2) an anchor for efficient orientation of the adsorbed TYR on the MLN-GCE, and (3) a stabilizer providing a suitable microenvironment for the adsorbed TYR on the MLN-GCE surface. This physical adsorption-based AO-coupled enzyme-modification strategy onto natural MLN would be a versatile strategy to develop cost-effective and environment-friendly natural mineral-based electrochemical biosensors and bioelectronic devices.
Recommended Citation
Y. Zhang et al., "Natural Molybdenite-And Tyrosinase-Based Amperometric Catechol Biosensor using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase," ACS Omega, vol. 6, no. 21, pp. 13719 - 13727, American Chemical Society, Jun 2021.
The definitive version is available at https://doi.org/10.1021/acsomega.1c00973
Department(s)
Mining Engineering
International Standard Serial Number (ISSN)
2470-1343
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2024 American Chemical Society, All rights reserved.
Creative Commons Licensing
This work is licensed under a Creative Commons Attribution 4.0 License.
Publication Date
01 Jun 2021
Comments
University of Science and Technology Liaoning, Grant 2019TD01