Thermal Induced Modification of the Contact Mechanics of Adhering Liposomes on Cationic Substrate
Abstract
The correlation between the mechanical property and the thermotropic transition of the phospholipid bilayer has been recently demonstrated (Chem. Phys. Lipids 110 (2001) 27). However, the role of thermal induced mechanical responses of phospholipid bilayer on the contact mechanics of liposome adhering on a cationic substrate has not been determined. In this study, confocal-reflectance interference contrast microscopy, phase contrast microscopy and contact mechanics modeling are applied to probe the adhesion mechanisms of liposomes in the presence of electrostatic interactions during the thermotropic transition of the lipid bilayer. When temperature increases from 23 to 49°C at pH 7.4, the degree of liposome deformation (a/R) and adhesion energy of dipalmitoyl-sn-glycero-3-phosphocholine liposome increases by 10% and remains constant, respectively, on 3-amino-propyl-triethoxy-silane (APTES) modified substrate. The extents of increase in these two parameters are highly dependent on the physicochemical properties of the rigid substrate. At pH 4, the adhesion energies above and below the phase transition temperature (Tm) are increased by one order of magnitude due to the formation of the free silanol groups on APTES substrate. In hypotonic condition, the degree of vesicle deformation remains constant and the adhesion energy reduces by 20% during sample heating. Under all conditions, the adhesion energy of the adhering liposome spans a few orders of magnitude against the increase of liposome size as the surface area to volume ratio is maximized in smallest vesicle. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Recommended Citation
V. Chan and K. Wan, "Thermal Induced Modification of the Contact Mechanics of Adhering Liposomes on Cationic Substrate," Chemistry and Physics of Lipids, vol. 120, no. 1 thru 2, pp. 131 - 143, Elsevier, Dec 2002.
The definitive version is available at https://doi.org/10.1016/S0009-3084(02)00124-X
Department(s)
Mechanical and Aerospace Engineering
Keywords and Phrases
Dipalmitoyl phosphatidyl-choline; Electrostatic interaction; Liposome adhesion; Thermal transition
International Standard Serial Number (ISSN)
0009-3084
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
01 Dec 2002
PubMed ID
12426082
Comments
Nantong University, Grant RG 15/00