Simultaneous Determination of Polyamines and Catecholamines in PC-12 Tumor Cell Extracts by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Abstract

A method to detect polyamines and catecholamines in PC-12 tumor cell extracts by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is described for the first time. Both derivatization conditions and buffer concentrations and pH were optimized. under optimized conditions the polyamines (putresine, spermine, spermidine) and catecholamines (dopamine, norepinephrine, epinephrine, serotonin) were derivatized with fluorescein isothiocyanate and separated at 25 kV in a fused-silica capillary (50 m ID×40 cm) with 0.1 M borate, pH 9.0, in less than 18 min. The influence of running buffer conditions, such as buffer pH and concentrations, were also investigated. Linearity of the analytes ranged from 0.05 to 1.0 mol/L, and the detection limit (S/N = 3 ) ranged from 0.03 to 2.50 nmol/L. The concentrations of polyamines and catecholamines in PC-12 tumor cell extracts were determined with this method.

Department(s)

Mechanical and Aerospace Engineering

Second Department

Chemistry

Keywords and Phrases

Adrenalin; Boric Acid; Catecholamine Derivative; Cell Extract; Dopamine; Fluorescein Isothiocyanate; Noradrenalin; Polyamine Derivative; Putrescine; Serotonin; Silicon Dioxide; Spermidine; Spermine; Animal Cell; Capillary Electrophoresis; Concentration (Parameters); Controlled Study; Derivatization; Fluorescence Analysis; Linear System; Nonhuman; PH; Process Optimization; Quantitative Analysis; Rat; Reproducibility; Sensitivity Analysis; Separation Technique; Tumor Cell; Animals; Catecholamines; Cell Extracts; Electrophoresis, Capillary; Hydrogen-Ion Concentration; PC12 Cells; Polyamines; Rats; Capillary electrophoresis; Catecholamines; Polyamines

International Standard Serial Number (ISSN)

0173-0835

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2004 John Wiley & Sons, All rights reserved.

Publication Date

01 Jun 2004

Link to Full Text

Share

 
COinS