Potential Toxicity of Bioactive Borate Glasses In-Vitro and In-Vivo

Abstract

Potential toxicity of a bioactive borate glass was evaluated using in-vivo animal models in soft tissue and bone and in-vitro cell culture using MLOA5 late osteoblast/early osteocytes cells. No toxicity was found between bioactive borate glass and subcutaneous tissue, in the liver, and only normal incidental changes in rat kidney. Bone growth across porous scaffolds composed of randomly oriented borate glass fibers was significantly higher than for a scaffold composed of a borosilicate or a silicate bioactive glass (13-93) fibers after 12 weeks in-vivo, p<0.05. In-vitro cell culture on bioactive glasses showed that under static culture conditions, borate glass disks tended to inhibit the growth of MLOA5 cells. The present work, along with literature data, show that bioactive borate glasses are biocompatible in-vivo and are not toxic to adjacent hard or soft tissues, or internal organs such as the kidney and liver, at the relatively high, estimated concentrations (<~126mg/kg/day). Based on the present results and literature data, ions released from the borate glasses such as alkali or boron were not toxic in a dynamic environment such as the body and should be considered for use in humans and other mammals for soft and hard tissue engineering applications.

Meeting Name

Advances in Bioceramics and Porous Ceramics V -- 36th International Conference on Advanced Ceramics and Composites (2012: Jan. 22- 27, Daytona Beach, FL)

Department(s)

Materials Science and Engineering

Second Department

Biological Sciences

Keywords and Phrases

Animal model; Dynamic environments; Hard tissues; Internal organs; Literature data; Porous scaffold; Static culture; Subcutaneous tissues; Bioceramics; Bone; Cell culture; Glass fibers; Silicates; Tissue; Tissue engineering; Toxicity; Bioactive glass

International Standard Book Number (ISBN)

978-1118205969

International Standard Serial Number (ISSN)

0196-6219

Document Type

Article - Conference proceedings

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2013 Wiley-Blackwell, All rights reserved.

Publication Date

01 Jan 2013

Link to Full Text

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