Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles

Author

Chuan-Chin Huang

Abstract

"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 μg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. Exposure to a sublethal concentration of ZnO increased the expression of BNIP, PRDX3, PRNP, and TXRND1 genes by at least or above 2.5 fold. These four genes are involved in apoptosis and oxidative stress responses"--Abstract, page iv.

Advisor(s)

Huang, Yue-wern

Committee Member(s)

Aronstam, Robert
Nam, Paul Ki-souk

Department(s)

Biological Sciences

Degree Name

M.S. in Environmental Biology

Comments

This work is supported by the Department of Biological Sciences and the cDNA Resource Center at the Missouri University of Science and Technology.

Publisher

Missouri University of Science and Technology

Publication Date

Summer 2009

Pagination

viii, 37 pages

Note about bibliography

Includes bibliographical references (pages 18-23).

Rights

© 2009 Chuan-Chin Huang, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Subject Headings

Calcium
Gene expression
Nanoparticles
Oxidative stress
Zinc oxide

Thesis Number

T 9541

Print OCLC #

472433673

Link to Catalog Record

Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.

Recommended Citation

Huang, Chuan-Chin, "Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles" (2009). Masters Theses. 93.
https://scholarsmine.mst.edu/masters_theses/93