Masters Theses
Abstract
"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 μg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. Exposure to a sublethal concentration of ZnO increased the expression of BNIP, PRDX3, PRNP, and TXRND1 genes by at least or above 2.5 fold. These four genes are involved in apoptosis and oxidative stress responses"--Abstract, page iv.
Advisor(s)
Huang, Yue-wern
Committee Member(s)
Aronstam, Robert
Nam, Paul Ki-souk
Department(s)
Biological Sciences
Degree Name
M.S. in Environmental Biology
Publisher
Missouri University of Science and Technology
Publication Date
Summer 2009
Pagination
viii, 37 pages
Note about bibliography
Includes bibliographical references (pages 18-23).
Rights
© 2009 Chuan-Chin Huang, All rights reserved.
Document Type
Thesis - Open Access
File Type
text
Language
English
Subject Headings
CalciumGene expressionNanoparticlesOxidative stressZinc oxide
Thesis Number
T 9541
Print OCLC #
472433673
Recommended Citation
Huang, Chuan-Chin, "Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles" (2009). Masters Theses. 93.
https://scholarsmine.mst.edu/masters_theses/93
Comments
This work is supported by the Department of Biological Sciences and the cDNA Resource Center at the Missouri University of Science and Technology.