Keywords and Phrases
Insect physiology; Metabolic cost of growth
“The metabolic cost of growth is the amount of energy invested to fuel the anabolic biochemical reactions of biosynthesis. It has been implicated in the efficiency of farm animals and fishes, thus, increased economic profit. The metabolic cost of growth in the cockroach nymph was reported to be 20-fold higher than that of the painted lady butterfly caterpillar. The cost-quality hypothesis was proposed to explain the extremely low biosynthesis energy cost in painted lady caterpillar when physiological and ecological processes the metabolic growth cost was attributed to could not. In this study, the cost-quality hypothesis was tested.
Midgut cells were isolated from the two insect species and the cell metabolic rates and growth rates were determined. Using the energy budget model, the metabolic cost of growth of the cells was estimated and compared to the values reported in the organisms. 7AAD viability staining and Annexin V apoptosis were used to determine cell viability and percentages of apoptotic cells in the insect species after exposure to various concentrations of oxidant. Cell viability and percentages of apoptotic cells were used as an index of cell quality.
The ratio of the estimated metabolic cost of growth between the caterpillar and the cockroach nymph at the cellular level is relatively equivalent to one obtained at the organismal level. The caterpillar cells were more vulnerable to oxidative damage than the cockroach, having lower cell viability and a higher percentage of apoptotic cells after treatment with oxidant. Therefore, the cost-quality hypothesis was established to be true”--Abstract, page iii.
Olbricht, Gayla R.
M.S. in Applied and Environmental Biology
Missouri University of Science and Technology
vii, 34 pages
© 2020 Taiwo Bolanle Iromini, All rights reserved.
Thesis - Open Access
Iromini, Taiwo Bolanle, "Link between energy investment in biosynthesis and cellular resistance to oxidative insult: Test the cost-quality hypothesis in insects" (2020). Masters Theses. 8003.