Masters Theses

Keywords and Phrases

DNA origami; Doxorubicin; Gene delivery; Gene editing; Triple negative breast cancer; Uptake

Abstract

"A review of gene delivery methods and gene editing methods, as well as original research utilizing DNA as a delivery vehicle is presented in the following thesis. Thousands of diseases have been linked to genes. Gene therapy, either delivering therapeutic genes or editing DNA bases, has arisen as a treatment option with the potential to cure diseases, rather than just ease symptoms. Genes and editing tools need to be delivered to cells for these therapies to be effective and many techniques have been developed to address the issue of delivery. Nonviral and viral methods have been used to deliver nucleic acids and several different protein systems have been employed to edit genes. Gene therapy will continue to evolve as delivery are improved. Along with being delivered as a therapeutic molecule, DNA has been investigated as a carrier itself. DNA origami, have been utilized to deliver chemotherapies to breast cancer. Globally, millions of women are affected by breast cancer each year. DNA origami was analyzed as a carrier for the chemotherapy Doxorubicin (DOX) in two triple negative breast cancer (TNBC) cell lines, a type of breast cancer with few treatments. The killing efficiency and uptake of DOX loaded into a model DNA origami triangle (DOX-DNA-T) were elucidated. Inhibition of various pathways revealed DOX-DNA-T was internalization by multiple energy-dependent pathways. DOX-DNA-T altered the subcellular localization of DOX and increased the concentration of DOX inside cells. A delayed killing was observed with DOX-DNA-T compared to free DOX, but the carrier was able to modulate the toxicity between cell lines. Overall, DNA delivery is able to treat various disease conditions and DNA origami is an interesting carrier for therapeutics"--Abstract, page iv.

Advisor(s)

Huang, Yue-Wern

Committee Member(s)

Wang, Risheng
Shannon, Katie

Department(s)

Biological Sciences

Degree Name

M.S. in Applied and Environmental Biology

Comments

The Biological Sciences Department, Missouri S&T Center for Research in Energy and Environment internal seed grant and the National Science Foundation provided fundng.

Research Center/Lab(s)

Center for Research in Energy and Environment (CREE)

Publisher

Missouri University of Science and Technology

Publication Date

Fall 2020

Journal article titles appearing in thesis/dissertation

  • Evolutionary Timeline of Genetic Delivery and Gene Therapy
  • DOX-loaded DNA Origami Triangle Uptake and Cytotoxic Effects in Triple Negative Breast Cancer Cell Lines

Pagination

xiv, 122 pages

Note about bibliography

Includes bibliographic references.

Rights

© 2020 Natalie Jordan Holl, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Thesis Number

T 11787

Electronic OCLC #

1240361921

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