Experimental studies on the kinetics of adsorption of beta-galactosidase interacting with immobilized anti-beta-galactosidase

Author

Barbara June Hearn

Abstract

"The adsorption of beta-galactosidase (BG) from solution by anti-beta-galactosidase monoclonal antibody (ABG) immobilized on nonporous, glass coated beads has been studied in a stirred batch adsorber at 4, 8, 15, and 20°C and for different initial BG concentrations. The elution of BG from the BG-ABG complex was also examined. The experiments performed in this work provided data from which information may be obtained about the influence of the concentration of BG and of the temperature on the dynamics of the adsorption of BG onto immobilized ABG, as well as on the total amount of BG adsorbed at equilibrium. Such experimental data may be used in studies involving the modelling of the kinetics of adsorption of BG onto immobilized ABG"--Abstract, page ii.

Advisor(s)

Liapis, Athanasios I.

Committee Member(s)

Findley, Marshall E., 1927-1991
Haddock, Glen

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

University of Missouri--Rolla

Publication Date

Summer 1989

Pagination

vii, 73 pages

Note about bibliography

Includes bibliographical references (pages 47-50).

Rights

© 1989 Barbara June Hearn, All rights reserved.

Document Type

Thesis - Restricted Access

File Type

text

Language

English

Subject Headings

Beta-galactosidase -- Absorption and adsorptionAdsorptionMolecular dynamicsChemical kinetics

Thesis Number

T 5895

Print OCLC #

20838363

Electronic OCLC #

921846276

Recommended Citation

Hearn, Barbara June, "Experimental studies on the kinetics of adsorption of beta-galactosidase interacting with immobilized anti-beta-galactosidase" (1989). Masters Theses. 774.
https://scholarsmine.mst.edu/masters_theses/774