Masters Theses

Keywords and Phrases

BMP-2; Calvarial Defect; Hydroxyapatite; Osteogenesis; Relaxin

Abstract

"Bone grafts are a common procedure for treating craniofacial injuries such as trauma, surgery, and reconstruction. Autografts and allografts have limitations which encourages the use of synthetic bone grafts. While synthetic bone grafts, such as hydroxyapatite (HA) microspheres, are advantageous for their cost and reproducibility, they lack the cells and growth factors needed for proper osteoinduction. Bone morphogenetic protein-2 (BMP-2) has been shown to increase the osteoinduction of grafts but the effective dose is above physiological levels and causes undesired effects on the body. Relaxin has been shown to enhance BMP-2's ability to induce bone formation in vitro and thus lower the dose of BMP-2 required for effective bone regeneration. In this study, HA microspheres sized 212-250µm were synthesized; the in vitro release of BMP-2 and relaxin into a medium of fetal bovine was measured. The release of both was slow and controlled and no hindrance to release was seen when both proteins were loaded on the microspheres. When implanted into rat calvarial defects for 6 weeks, an enhancement to new bone percent was seen when loading HA microspheres with 0.5µg BMP-2 and 0.05µg of relaxin compared to microspheres loaded with 0.5µg BMP-2 alone. A 50% reduction in BMP-2 dose, compared to a previously published dose, was seen when relaxin (at 0.05, 0.1, and 0.25µg per defect) was loaded on the HA microspheres. A 75% reduction in BMP-2 dose was seen when 0.05µg per defect of relaxin was loaded. These results suggest that a combination of relaxin and BMP-2 loaded onto HA microspheres will reduce the dosage of BMP-2 required to provide significant bone regeneration."--Abstract, page iii.

Advisor(s)

Huang, Yue-Wern

Committee Member(s)

Rahaman, M. N., 1950-
Semon, Julie A.

Department(s)

Biological Sciences

Degree Name

M.S. in Applied and Environmental Biology

Sponsor(s)

National Institutes of Health (U.S.)
Missouri University of Science and Technology. Center for Biomedical Research

Publisher

Missouri University of Science and Technology

Publication Date

Fall 2017

Pagination

x, 39 pages

Note about bibliography

Includes bibliographical references (pages 35-38).

Rights

© 2017 Sahitya Injamuri, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Thesis Number

T 11224

Electronic OCLC #

1021857438

Download

Share

 
COinS