Masters Theses

Abstract

"A strain of Thiobaci.llus thiooxidans was isolated from a West Virginia coal mine drainage. This strain was characterized by its production of sulfuric acid in shake flasks. The strain was also compared with a pure culture of h thiooxidans obtained from the American Type Culture Collection (ATCC number 8085).

The strain was grown in submerged culture in a 16-liter pilot scale fermenter. Two aeration systems, air and air enriched with carbon dioxide, were examined. The air enriched with carbon dioxide gave a much more rapid growth rate. The culture reached the mid-log phase of growth after 80 hours of incubation as compared with 100 to 110 hours using air alone. The culture time using carbon dioxide enriched air was reduced to 80 hours from the reported (4) 264 hours in stationary flasks OD 168 hours in shake flasks The cell yield was increased to 1.0 gram per liter wet weight (estimated) as compared to 0.30 grams wet weight per liter. The latter is the rnaximum cell yield reported in the literature (52)"-- Abstract p. ii

Advisor(s)

Siehr, Donald J.

Committee Member(s)

Wellek, Robert M.
Grigoropoulis, Sotorios G.
Scrivner, Jack M., 1929-2004

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

University of Missouri at Rolla

Publication Date

1964

Pagination

vi, 86 pages

Note about bibliography

Includes bibliographical references (pages 73-84)

Rights

© 1964 Edward Hibbert Crum, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Thesis Number

T 1614

Print OCLC #

5959294

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