Masters Theses

Yinan Lin

Keywords and Phrases

13-93 bioactive glass; 3D printing; Angiogenesis; BMP2; In vivo; Osteogenesis

Abstract

"The main objective of this project was to evaluate the capacity of strong porous silicate (13-93) bioactive glass scaffolds prepared by a robocasting technique to regenerate bone and stimulate angiogenesis in a rat calvarial defect model. The scaffolds were created with the same grid-like microstructure but in a variety of formulations: (i) as-fabricated, (ii) pretreated in an aqueous phosphate solution to convert the glass surface to hydroxyapatite, (iii) loaded with bone morphogenetic protein-2 (BMP2) (1 µg per scaffold), and (iv) doped with copper (0.4-2.0 wt. % CuO). When compared to the as-fabricated scaffolds, the pretreated scaffolds enhanced bone regeneration at 6 weeks but not at 12 or 24 weeks. In comparison, the BMP2-loaded scaffolds enhanced bone regeneration at all three implantation times and they were almost completely infiltrated with lamellar bone within 12 weeks. Doping the as-fabricated scaffolds with 0.4 and 0.8 wt. % CuO did not affect the response of preosteoblastic MC3T3-E1 cells in vitro and bone regeneration in vivo. In comparison, the scaffolds doped with 2.0 wt. % CuO were toxic to the cells in vitro and significantly inhibited bone regeneration at 6 weeks post-implantation. The area of new blood vessels in the new bone that infiltrated the scaffolds at 6 weeks post-implantation was significantly enhanced by the BMP2 loading but not by CuO dopant concentrations of 0.4 and 0.8 wt. %. The fibrous tissue that infiltrated the scaffolds doped with 2.0 wt. % CuO showed a significantly higher blood vessel area than the as-fabricated scaffolds. Loading silicate 13-93 bioactive glass scaffolds with BMP2 significantly improved their capacity to regenerate bone in rat calvarial defects at 6 weeks post-implantation when compared to doping the glass with copper"--Abstract, page iv.

Advisor(s)

Rahaman, M. N., 1950-

Committee Member(s)

Brow, Richard K.
Bal, Sonny B.

Department(s)

Materials Science and Engineering

Degree Name

M.S. in Materials Science and Engineering

Publisher

Missouri University of Science and Technology

Publication Date

Fall 2015

Journal article titles appearing in thesis/dissertation

  • Long-term bone regeneration, mineralization and angiogenesis in rat calvarial defects implanted with strong porous bioactive glass (13-93) scaffolds
  • Response of osteoblastic MC3T3-E1 cells and osseous defects to strong porous scaffolds of silicate 13-93 bioactive glass doped with copper

Pagination

xii, 88 pages

Note about bibliography

Includes bibliographical references.

Rights

© 2015 Yinan Lin, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Subject Headings

Bone regenerationTissue scaffoldsBioactive compoundsGlass in medicineThree-dimensional printing

Thesis Number

T 10790

Electronic OCLC #

936208262

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