Masters Theses

Keywords and Phrases

Glass microfibers and bead; SKH1 mice; Skin fold imaging

Abstract

"The primary objective of this research project was to evaluate the effects of three different compositions of bioactive glass microfibers (45S5, 13-93B3, and 13-93B3Cu) and bioactive glass beads (13-93, 13-93B3, and 13-93B3Cu) on angiogenesis in subcutaneous tissue in the SKH1 'hairless' mouse. Microvascular responses to the bioactive glass implants were investigated via three experimental approaches: noninvasive vital imaging of microvasculature in dorsal skin windows; quantitative histomorphometry of microvascular densities; and quantitative PCR measurements of mRNA expression of pro-angiogenic cytokines VEGF and FGF-2. The live imaging of dorsal skin window preparations in the hairless SKH1 showed the formation of a halo-like structure infused with vessels in soft tissue surrounding borate-based 13-93B3 and 13-93B3Cu glass beads two weeks after implantation. This response was not observed around silicate-based 13-93 glass beads. Quantitative histomorphometry of tissue implanted with 45S5, 13-93B3, and 13-93B3Cu glass microfiber plugs revealed microvascular densities that were 1.6-, 2.3-, and 2.7-fold higher, respectively compared to the sham control tissues whereas the 13-93, 13-93B3, and 13-93B3Cu glass beads produced a 1.3-, 1.6-, and 2.5-fold increase, respectively, compared to the sham control. Quantitative PCR measurements indicate a marginally significant increase in expression of VEGF mRNA in skin tissues with 13-93B3Cu. This latter outcome supports the project hypothesis that 13-93B3Cu glass induces VEGF expression followed by neovascularization, a key process of wound healing."--Abstract, page iv.

Advisor(s)

Brown, Roger F.

Committee Member(s)

Day, D. E.
Rahaman, M. N., 1950-

Department(s)

Biological Sciences

Degree Name

M.S. in Applied and Environmental Biology

Publisher

Missouri University of Science and Technology

Publication Date

Spring 2014

Journal article titles appearing in thesis/dissertation

  • In vivo evaluation of angiogenic effect of borate glass in the SKH1 hairless mouse

Pagination

x, 63 pages

Note about bibliography

Includes bibliographical references (pages 38-43).

Rights

© 2014 Richard Watters, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Subject Headings

Neovascularization
Glass beads
Bioactive compounds -- Analysis
Vascular endothelial growth factors

Thesis Number

T 10489

Electronic OCLC #

882552795

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