Masters Theses
Abstract
"The enzyme indole acetic acid oxidase is produced by the fungus Hygrophorous conicus when it is grown in the presence of indole acetic acid (IAA) or tryptamine. The IAA acts both as an inducer and a substrate for this intracellular enzyme. The product of the oxidation catalyzed by the IAA oxidase is oxindole acetic acid (OIAA).
The rate of growth and the rate of formation of the enzyme IAA oxidase by H.conicus were studied in different media. Of these, a nitrate medium containing 0.5 per cent Yeast extract (Y.E.) was found to be the best for the production of H.conicus mycelium. A maltose medium was better than the other media tested so far as the growth of H,conicus and the formation of the enzyme IAA oxidase were concerned, The addition of 0.5 per cent Y.E. to any of the media tested suppressed the formation of the enzyme IAA oxidase of H.conicus.
The Krebs cycle enzyme succinic dehydrogenase was found in the particulate fraction, sedimented at 24,000 x g for 20 minutes from a cell-free preparation of H,conicus.
The use of sonic oscillations for 10 minutes at 4-5⁰C was found to solubilize, to some extent, the enzyme succinic dehydro genase of H,conicus.
The phenazine methosulfate method for the assay of succinic dehydrogenase activity gave higher QO₂(N) values than the cytochrome c method"--Abstract, pages ii-iii.
Advisor(s)
Siehr, Donald J.
Committee Member(s)
Grigoropoulos, Sotirios G.
Kincannon, Donny F.
Hanna, Samir B.
Department(s)
Chemistry
Degree Name
M.S. in Chemistry
Publisher
University of Missouri at Rolla
Publication Date
1966
Pagination
viii, 90 pages
Note about bibliography
Includes bibliographical references (pages 67-70).
Rights
© 1966 Jagdish M. Mehta, All rights reserved.
Document Type
Thesis - Open Access
File Type
text
Language
English
Subject Headings
Succinate dehydrogenaseIndoleacetic acidKrebs cycleMitochondriaOxidases
Thesis Number
T 1841
Print OCLC #
5972964
Electronic OCLC #
896725639
Recommended Citation
Mehta, Jagdish M., "The activity of succinic dehydrogenase and indole acetic acid oxidase in cell-free preparations of Hygrophorous conicus" (1966). Masters Theses. 5728.
https://scholarsmine.mst.edu/masters_theses/5728
