Masters Theses

Keywords and Phrases

45S5; 13-93; hydroxyapatite; in-vitro; in-vivo; bone scaffolds

Abstract

"For nearly 40 years, glasses with compositions characterized as 'bioactive' have been studied for uses in bone regeneration and growth. The first glass developed, and easily the most studied bioactive glass, is known as silicate based 45S5. This glass is currently used in various forms, most notably particulate form, to treat bone defects. Loose particulate of 45S5 is most often used for medical use because the relatively low silica content (45wt%) makes sintering difficult due the glasses tendency to crystallize when heated to temperatures typical of softening glass (>Tg).

Another bioactive glass more recently developed has characteristics more favorable for secondary manufacturing than 45S5, i.e. sintering and formation of continuous glass fibers, and is known as silicate based 13-93. Although 13-93 is slower to react with body fluids to form hydroxyapatite (HA) than 45S5, the benefits of increased sintering and processing capabilities for producing three dimensional interconnected porous structures has gained it attention.

Three dimensional scaffolds comprised of bioactive 13-93 and 45S5 melt derived glass fibers were studied to determine if the reactivity of the glasses had any effect on the attachment and growth of pre-osteoblast cells through in-vitro culturing. Additional bioactive assessment of the scaffolds was completed by an in-vivo evaluation for the formation of new bone through subcutaneous implantation of mesenchymal stem cell seeded and un-seeded scaffolds. The growth indicated by DNA analysis of the in-vitro cell seeded model and the formation of new bone in both scaffolds tested in-vivo show promise for one day using sintered glass fiber scaffolds for bone tissue regeneration in humans"--Abstract, page iv.

Advisor(s)

Day, D. E.

Committee Member(s)

Brown, Roger F.
Brow, Richard K.
Rahaman, M. N., 1950-

Department(s)

Materials Science and Engineering

Degree Name

M.S. in Ceramic Engineering

Publisher

University of Missouri--Rolla

Publication Date

Spring 2007

Journal article titles appearing in thesis/dissertation

  • Scaffold Characterization and In-vitro Analysis
  • Comparison of Three-Dimensional Bioactive Glass Fiber Scaffolds After In-vivo Implantation in Rats

Pagination

xiv, 92 pages

Note about bibliography

Includes bibliographical references.

Rights

© 2007 Steven Barrett Jung, All rights reserved.

Document Type

Thesis - Restricted Access

File Type

text

Language

English

Subject Headings

Biomedical materials -- EvaluationBone regenerationBones -- GrowthHydroxyapatite

Thesis Number

T 9133

Print OCLC #

173661277

Electronic OCLC #

905853502

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