Oxidation of trichloroethylene by microsomal cytochrome P450 enzymes

Author

Arun Sahu

Abstract

"Trichloroethylene (TCE) is a common ground water contaminant which is used extensively as industrial reactant and in cleaners, degreasers and certain paint thinners. TCE is probably a carcinogen to humans (Group 2A, American Cancer Society) whereas it is metabolized by Cytochrome P450 to metabolites which are known carcinogens for rodents and other animal models. A panel of human P450 enzymes expressed as cDNAs in microsomes isolated from Baculovirus-infected insect cells 'supersomes', (human liver CYPs 2E1, 2C9*1 and 2B6) was used to study the metabolite profile of the enzymatic reaction with various concentrations of TCE (0.4 - 4.0 mM) in Tris buffer (pH 7.4) in the presence of NADPH. The activity profile for the three Supersomes are found to be in the order of 2E1 > 2B6 > 2C9*1. The major products detected were chloral, glyoxylic acid, carbon monoxide, formic acid and dichloroacetic acid with traces of trichloroacetic acid and no detectable trichloroethanol. Small amounts of TCE oxide were also observed which can rearrange to form carbon monoxide, formic acid, glyoxylic acid and dichloroacetic acid. The rearrangement products of TCE are consistent with the data found in the literature"--Abstract, page iii.

Department(s)

Chemistry

Degree Name

M.S. in Chemistry

Publisher

University of Missouri--Rolla

Publication Date

Fall 2005

Pagination

xiv, 95 pages

Rights

© 2005 Arun Sahu, All rights reserved.

Document Type

Thesis - Citation

File Type

text

Language

English

Subject Headings

Trichloroethylene -- OxidationCytochrome P-450

Thesis Number

T 8877

Print OCLC #

70784097

Recommended Citation

Sahu, Arun, "Oxidation of trichloroethylene by microsomal cytochrome P450 enzymes" (2005). Masters Theses. 3824.
https://scholarsmine.mst.edu/masters_theses/3824