Masters Theses
Abstract
"There has been little research looking at the degree of antibiotic resistance and the disinfection of the bacteria associated with animal wastes generated at concentrated animal feeding operations (CAFOs). To address this concern, the diversity of tetracycline resistance was evaluated by screening tetracycline resistant isolates for the presence of 18 different genes that confer tetracycline resistance. Forty-eight of 85 isolates were found to not contain targeted tet efflux genes. None of the isolates possessed any of the targeted ribosomal protection proteins. The disinfection potential of chlorine and ultraviolet (UV) light against aerobic lagoon bacteria was examined. Furthermore, the role of solids in the lagoon was investigated as a potential barrier to disinfection. It was found that 30 mg/L of chlorine achieved a 2-4 log removal of bacteria in all lagoon samples. Increasing the amount of chlorine provided little additional disinfection activity. Chlorine was equally or more effective against bacteria resistant to either chlorotetracycline, lincomycin, sulfamethazine, or tetracycline. The inability to achieve complete disinfection could be due to the presence of two potentially chlorine resistant bacterial isolates, which most closely related to Bacillus subtilis and }Jacillus lichenifonnis. A UV dose of 3240 mJ/cm2 effectively dropped the number of bacteria in all samples to approximately 1000 cfu/ml. The highest UV dosage of 9720 mJ/cm2 essentially drove the number of bacteria to zero. Our results clearly show there are still unknown genes that confer tetracycline resistance and that most facultative bacteria in swine waste lagoons can be effectively treated using chlorine or UV light. Thus, it is critical to understand gene-mediated resistance as well as the antibiotic resistant organisms and their potential control through disinfection"-- Abstract, p. iii
Advisor(s)
Mormile, Melanie R.
Adams, Craig D.
Committee Member(s)
Westenberg, David J.
Department(s)
Biological Sciences
Degree Name
M.S. in Applied and Environmental Biology
Publisher
University of Missouri--Rolla
Publication Date
Spring 2005
Pagination
viii, 57 pages
Note about bibliography
Includes bibliographical references (pages 53-56)
Geographic Coverage
United States
Rights
© 2005 John J. Macauley, All rights reserved.
Document Type
Thesis - Restricted Access
File Type
text
Language
English
Subject Headings
Swine farrowing facilities -- Environmental aspects -- United StatesDisinfection and disinfectantsChlorineAntibioticsTetracycline
Thesis Number
T 8774
Print OCLC #
62675810
Recommended Citation
Macauley, John J., "Diversity of tetracycline resistant bacterial isolates and disinfection treatment of swine lagoons" (2005). Masters Theses. 3716.
https://scholarsmine.mst.edu/masters_theses/3716
Share My Thesis If you are the author of this work and would like to grant permission to make it openly accessible to all, please click the button above.