Abstract
Hedgehog signaling is crucial for vertebrate development and physiology. Gli2, the primary effector of Hedgehog signaling, localizes to the tip of the primary cilium, but the importance of its ciliary localization remains unclear. We address the roles of Gli2 ciliary localization by replacing endogenous Gli2 with Gli2ΔCLR, a Gli2 variant not localizing to the cilium. The resulting Gli2ΔCLRKI and Gli2ΔCLRKI;Gli3 double mutants resemble Gli2-null and Gli2;Gli3 double mutants, respectively, suggesting the lack of Gli2ΔCLR activation in development. Significantly, Gli2ΔCLR cannot be activated either by pharmacochemical activation of Smo in vitro or by loss of Ptch1 in vivo. Finally,Gli2ΔCLR exhibits strong transcriptional activator activity in the absence of Sufu, suggesting that the lack of its activation in vivo results from a specific failure in relieving the inhibitory function of Sufu. Our results provide strong evidence that the ciliary localization of Gli2 is crucial for cilium-dependent activation of Hedgehog signaling.
Recommended Citation
J. Liu et al., "The Loss of Hh Responsiveness by a Non-Ciliary Gli2 Variant," Development (Cambridge), vol. 142, no. 9, pp. 1651 - 1660, Company of Biologists Ltd, Mar 2015.
The definitive version is available at https://doi.org/10.1242/dev.119669
Department(s)
Engineering Management and Systems Engineering
Second Department
Biological Sciences
Keywords and Phrases
Cilium; Hedgehog signaling; Mouse; Spinal cord; Sufu
International Standard Serial Number (ISSN)
0950-1991; 1477-9129
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2015 Company of Biologists Ltd, All rights reserved.
Publication Date
01 Mar 2015
PubMed ID
25834022
