Detection of Lipid Bilayer Membranes Formed on Silica Fibers by Double-long Period Fiber Grating Laser Refractometry
Abstract
The formation of lipid bilayer membranes on silica optical fiber surfaces is critical to the development of new optical biosensors based on supported lipid bilayer membrane technology. This paper reports on a new long period fiber grating (LPFG) method using double gratings, one serving as the sensor and another as the reference to correct for environmental factors, to study supported lipid membranes on a silica optical fiber. a method for correcting the effect of the environmental variation on sensor responses is described. the measurements show that formation of eggPC lipid bilayer membranes on the silica optical fiber surface takes place in about 3 min, which is accompanied with about a 500 picometer decrease in the resonance coupling wavelength of the sensor grating. the formation process of eggPC lipid bilayer membranes with and without Gramicidin was measured. It was found that while the presence of Gramicidin does not affect the rate of lipid bilayer membrane formation on the silica fiber, it causes a greater decrease in the resonance coupling wavelength than pure eggPC because of the increased film refractive index. Formation of a lipid bilayer membrane on the silica optical fiber surface was verified by confocal microscopy and through fluorescence recovery after photobleaching (FRAP) analysis, confirming the formation of a single lipid bilayer on the surface of the silica fiber with a diffusion coefficient of 1.2 μ m 2 s-1. © 2010 Elsevier B.V.
Recommended Citation
C. L. Eggen et al., "Detection of Lipid Bilayer Membranes Formed on Silica Fibers by Double-long Period Fiber Grating Laser Refractometry," Sensors and Actuators, B: Chemical, vol. 150, no. 2, pp. 734 - 741, Elsevier, Oct 2010.
The definitive version is available at https://doi.org/10.1016/j.snb.2010.08.010
Department(s)
Electrical and Computer Engineering
Keywords and Phrases
Optical biosensing; Refractometry; Supported lipid bilayer membranes; Vesicle deposition
International Standard Serial Number (ISSN)
0925-4005
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
28 Oct 2010
Comments
Office of Naval Research, Grant N00014-07-0008