Abstract

Utilizing a simple fluidic structure, we demonstrate the improved performance of oxidase-Based enzymatic biosensors. Electrolysis of water is utilized to generate bubbles to manipulate the oxygen microenvironment close to the biosensor in a fluidic channel. for the proper enzyme reactions to occur, a simple mechanical procedure of manipulating bubbles was developed to maximize the oxygen level while minimizing the pH change after electrolysis. the sensors show improved sensitivities based on the oxygen dependency of enzyme reaction. in addition, this oxygen-rich operation minimizes the ratio of electrochemical interference signal by ascorbic acid during sensor operation (i.e., amperometric detection of hydrogen peroxide). Although creatinine sensors have been used as the model system in this study, this method is applicable to many other biosensors that can use oxidase enzymes (e.g., glucose, alcohol, phenol, etc.) to implement a viable component for in-line fluidic sensor systems. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Department(s)

Electrical and Computer Engineering

Second Department

Chemical and Biochemical Engineering

Publication Status

Open Access

Comments

National Institute of Biomedical Imaging and Bioengineering, Grant R15EB006611

Keywords and Phrases

Biosensor; Bubble; Electrolysis; Hydrogen; Oxidase; Oxygen

International Standard Serial Number (ISSN)

1424-8220

Document Type

Article - Journal

Document Version

Final Version

File Type

text

Language(s)

English

Rights

© 2024 The Authors, All rights reserved.

Creative Commons Licensing

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Publication Date

01 Jul 2012

PubMed ID

23012527

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