Automatic Karyotyping of Metaphase Cells with Overlapping Chromosomes

Abstract

Image analysis research aimed at analyzing images of banded, human chromosomes is described. An experimental automated karyotyping system (AKS) has been assembled. The goal of the system is to label the chromosomes from a metaphase image with minimal human intervention. The AKS is unique in that it is designed to automatically process cells containing overlapping chromosomes. It also has four novel algorithmic features: the use of cross section sequence graphs, an over-segmentation-based segmentation strategy, the use of the 'pale path' concept for banded chromosomes and the application of mathematical programming for cell-level chromosome classification in cells with overlapping chromosomes. System performance was analyzed using a variety of metaphase images exhibiting various levels of overlapping chromosomes.

Department(s)

Electrical and Computer Engineering

Keywords and Phrases

Algorithm; Autoanalysis; Chromosome Analysis; Human; Human Cell; Image Analysis; Karyotype; Karyotyping; Mathematical Model; Metaphase Chromosome; Priority Journal; Algorithms; Benchmarking; Chromosome Banding; Chromosomes; Humans; Image Processing; Computer-assisted; Metaphase; Sex Chromosomes; Software; Automated Karyotyping System; Cross Section Sequence Graph; Neural Network; Over-segmentation; Pale Path

International Standard Serial Number (ISSN)

0010-4825

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1999 Elsevier, All rights reserved.

Publication Date

01 Jan 1999

Link to Full Text

Share

 
COinS