The Effects of Image Manipulation on Automated Karyotyping

Abstract

Karyotyping is one of the standard tools for human genetic investigations. Karyotyping involves the classification and interpretation of chromosomes found in a metaphase spread. As part of the automated karyotyping process, image manipulation is required for appropriately positioning metaphase spread chromosomes in their corresponding karotype. Image manipulation, specifically image rotation, reorganizes the grey level information within chromosomes to facilitate analysis. Statistical tests are performed to compare features related to banding pattern and length between unmanipulated chromosomes and corresponding rotated chromosomes. Based on experimental results, reorganizing the grey-level information yields statistically different chromosome features. Depending on the purpose of chromosome image analysis, the interpretation process of karyotyping could be impaired with chromosome feature distortion due to image rotation.

Department(s)

Electrical and Computer Engineering

Keywords and Phrases

Automation; Camera; Chromosome Banding Pattern; Computer; Genetic Analysis; Human; Image Processing; Karyotyping; Metaphase; Statistical Analysis; Image Enhancement; Computer-assisted; Automated Karyotyping; Human Chromosomes; Medical Imaging

International Standard Serial Number (ISSN)

0067-8856

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1997 ISA - Instrumentation, Systems, and Automation Society, All rights reserved.

Publication Date

01 Feb 1997

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