Doctoral Dissertations

Keywords and Phrases

Biospecific Adsorption; Affinity Chromatography; Experimental and Theoretical Data; Single and Multicomponent; Monovalent and Multivalent Adsorbates; Fixed Bed Operation; Periodic Countercurrent Operation

Abstract

"A general model is presented and used to predict the dynamic behavior of the adsorption, wash, and elution stages of biospecific adsorption (affinity chromatography) in finite bath and column operations. The model accounts for film and pore diffusional mass transfer resistances and the rates of interaction between adsorbates and ligands. For column operations the effect of axial dispersion in the flowing fluid stream is included. The model is applicable to single and multi-component biospecific as well as non-specific adsorption, and the adsorbates may be monovalent or multivalent. In the elution stage both non-selective and selective elution of monovalent adsorbates is considered.

The predictions of the model are compared with the experimental batch data of the adsorption of ß-galactosidase onto anti-ß-galactosidase. The results indicate that the formation of the ß-galactosidase anti-ß-galactosidase complex can be described by a second order reversible interaction rate expression. An information flow diagram is presented and indicates that one may predict the behavior of biospecific adsorption in a column only from experimental batch data and appropriate batch and column models.

Certain characteristic parameters which influence significantly the behavior of biospecific adsorption have been identified and include the Sherwood, Porath, and the adsorbate-ligand dissociation parameters. The results of biospecific adsorption of bivalent adsorbates indicate that a competition for ligands occurs between molecules forming one-site interaction and two-site interaction complexes. This competition can lead to the displacement of the adsorbate from the adsorbate-ligand complex whose formation is least favored. For the wash stage in a batch system more than one wash may be required to satisfy a specified high level of product purity, and the number of washes, overall wash time, and the amount of product lost during the wash stage depend on the criterion used to terminate each wash. The time duration of the elution stage is significantly influenced by the rate of desorption and the Sherwood number of the adsorbate. The amount of adsorbate recovered during selective elution in a batch system is greatly influenced by the initial concentration of the eluent and the equilibrium dissociation constants of the adsorbate-ligand and adsorbate-eluent complexes.

The results of fixed bed operation show that the time of breakthrough of the adsorbate is significantly influenced by the rate of the adsorbate-ligand interaction. The occurrence of early breakthrough in systems where the adsorbate-ligand interaction is finite may lead to a very low utilization of the immobilized ligands. The model simulations of periodic countercurrent operation indicate that when a single column is divided into two beds, the ligand utilization can be almost four times higher than for single columns of the same bed length"--Abstract, pages iv-v.

Advisor(s)

Liapis, Athanasios I.

Committee Member(s)

Crosser, Orrin K.
Neogi, P. (Partho), 1951-
Poling, Bruce E.

Department(s)

Chemical and Biochemical Engineering

Degree Name

Ph. D. in Chemical Engineering

Sponsor(s)

National Science Foundation (U.S.

Comments

This work was supported in part by the National Science Foundation under Grant No. CBT-8416165.

Publisher

University of Missouri--Rolla

Publication Date

Fall 1986

Journal article titles appearing in thesis/dissertation

  • The modeling and analysis of biospecific adsorption in a finite bath - adsorption and wash stages
  • The modeling and analysis of the elution stage of biospecific adsorption in a finite bath
  • The modeling and analysis of biospecific adsorption in fixed and periodic countercurrent beds - Adsorption and wash stages
  • The modeling and analysis of biospecific adsorption in a fixed bed - Elution stage

Pagination

xxix, 280 pages

Note about bibliography

Includes bibliographical references.

Rights

© 1986 Bo Henrik Arve, All rights reserved.

Document Type

Dissertation - Open Access

File Type

text

Language

English

Subject Headings

Affinity ChromatographyAdsorption -- Mathematical modelsDiffusion -- Mathematical modelsSeparation (Technology)Mass transferMacromolecules

Thesis Number

T 5440

Print OCLC #

16769563

Electronic OCLC #

930382254

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