Doctoral Dissertations
Abstract
"The activity of the enzyme succinic dehydrogenase (succinate : phenazine methosulfate oxidoreductase, EC 1.3.99.1) of Hygrophorus conicus, a member of a class of fungi known as basidiomycetes, was found to be stimulated by nitrate. Two substances were tested as electron acceptors in the measurement of succinic dehydrogenase activity. Of the two substances tried, phenazine methosulfate and brilliant cresyl blue, the former was found to be the best. Nicotinamide adenine dinucleotide (NAD⁺) was a necessary cofactor for the enzyme and could be readily dissociated from the enzyme. The enzyme has the properties of a high molecular weight nucleo- or lipoprotein.
The presence of two kinds of succinic dehydrogenase in H.conicus was demonstrated. One was precipitated at 10% ammonium sulfate saturation and the other at 40%...
The oxidation of IAA by the enzyme IAA oxidase of H.conicus produces at least two more products besides oxindole acetic acid. Attempts to separate and characterize these products were unsuccessful. It is suggested that one of the products has a close structural relationship with o-formaminobenzoyl acetic acid while the other may be 5-hydroxy oxindole acetic acid.
It was concluded that H.conicus has a great facility for detoxification of toxic substances present in the medium by either stimulating or inducing oxidative enzymes"--Abstract, page iii-iv.
Advisor(s)
Siehr, Donald J.
Committee Member(s)
Webb, William H.
Stoffer, James O.
Campbell, Benedict
Nicholson, Larry M.
Department(s)
Chemistry
Degree Name
Ph. D. in Chemistry
Sponsor(s)
National Science Foundation (U.S.)
Publisher
University of Missouri--Rolla
Publication Date
1968
Pagination
xii, 123 pages
Note about bibliography
Includes bibliographical references (pages 109-114).
Rights
© 1968 Jagdish M. Mehta, All rights reserved.
Document Type
Dissertation - Open Access
File Type
text
Language
English
Subject Headings
Cytochrome cDenitrifying bacteriaIndoleacetic acidSuccinate dehydrogenase
Thesis Number
T 2148
Print OCLC #
5998994
Electronic OCLC #
794113109
Recommended Citation
Mehta, Jagdish M., "A study of succinic dehydrogenase, indole acetic acid oxidase, nitrate reductase and cytochrome c reductase in Hygrophorus Conicus" (1968). Doctoral Dissertations. 1875.
https://scholarsmine.mst.edu/doctoral_dissertations/1875
