Doctoral Dissertations

Abstract

"The objectives of this investigation were: (a) to culture large quantities of Thiobacillus thiooxidans cells, (b) to find an acceptable method of rupturing the cells, (c) to find an acceptable method of isolating the cell walls, and (d) to analyze the cell wall components, especially the amino acid and sugar components. The strain of T. thiooxidans used for this investigation was American Type Culture Collection Number 8085. The surface active agents Tergitol 08 and Tween 80 were investigated for their ability to reduce the lag period found in the growth of T. thiooxidans. Although a concentration of 12.5 ppm Tween 80 did decrease the lag period, the use of these surfactants was not effective in increasing the amount of cells grown over a specific time period because they reduced the cell yield. Two cell rupture techniques, sonication and pressure cell rupture, coupled with either washing with deionized water or washing treatment with IR-120 and AG-1X2 ion exchange resins, were tested"--Abstract, page ii-iii.

Advisor(s)

Siehr, Donald J.

Committee Member(s)

Peterson, John E.
Grigoropoulos, Sotirios G.
Grice, Harvey H., 1912-1993
Wellek, Robert M.

Department(s)

Chemical and Biochemical Engineering

Degree Name

Ph. D. in Chemical Engineering

Publisher

University of Missouri at Rolla

Publication Date

1967

Pagination

ix, 102 pages

Note about bibliography

Includes bibliographical references (pages 92-100).

Rights

© 1967 Edward Hibbert Crum, All rights reserved.

Document Type

Dissertation - Open Access

File Type

text

Language

English

Subject Headings

Amino acidsMonosaccharidesThiobacillus thiooxidans

Thesis Number

T 2036

Print OCLC #

18253580

Electronic OCLC #

780619622

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