The microbial hydrolysis of cellulose and some factors affecting the production of cellulase in a submerged culture of Alternaria sp

Author

Robert Murray Logan

Abstract

"A mile hydrolyzing procedure was utilized to degrade oak sawdust. Its capability of producing a suitable fermentatation [sic] medium was studied. In the interest of solubilizing, enzymatically, the carbohydrate portion of oak sawdust, an investigation was conducted in which various microorganisms were examined for their ability to utilize different forms of cellulose for their growth and to produce cellulose decomposing enzymes. The microorganisms included a myxobacterium, several fungi, and a wood-rotting basidiomycete. The fungi included two highly cellulolytic organisms, Myrothecium verrucaria and Trichoderma viride. The myxobacterium used was Sorangium cellulosum and the basidiomycete was Pleurotus ostreatus. The principla substrates employed in the investigation were swollen cellulose, filter paper, and oak sawdust. An unidentified species of Alternaria was isolated. Its culture characteristics and cellulase producing ability were determined and compared with those of Myrothecium verrucaria. The hydrolyzing power of a commercial cellulase preparation, Cellulase 35* (*Registered trademark of the Rohm and Haas Company), was compared with the hydrolyzing power of cell-free extracts from cultures of Alternaria sp. Detailed culture investigations with Alternaria sp were conducted to determine the best substrate, pH, temperature, and degree of agitation for the production of enzymes capable of hydrolyzing swollen cellulose. The hydrolysis products resulting from the action of Alternaria sp cellulase were qualitatively and quantitatively determined"--Abstract, page ii-iii.

Advisor(s)

Siehr, Donald J.

Committee Member(s)

White, [illegible]
Grigoropoulos, Sotirios G.
Grice, Harvey H., 1912-1993
Strunk, Mailand R., 1919-2008

Department(s)

Chemical and Biochemical Engineering

Degree Name

Ph. D. in Chemical Engineering

Publisher

University of Missouri at Rolla

Publication Date

1965

Pagination

xi, 179 pages

Note about bibliography

Includes bibliographical references (pages 165-172).

Rights

© 1965 Robert M. Logan, All rights reserved.

Document Type

Dissertation - Open Access

File Type

text

Language

English

Subject Headings

AlternariaCellulaseCelluloseHydrolysisPlant cell culture

Thesis Number

T 1787

Print OCLC #

5969009

Electronic OCLC #

805424653

Recommended Citation

Logan, Robert Murray, "The microbial hydrolysis of cellulose and some factors affecting the production of cellulase in a submerged culture of Alternaria sp" (1965). Doctoral Dissertations. 1869.
https://scholarsmine.mst.edu/doctoral_dissertations/1869