Purification of human antibody from transgenic corn using aqueous two-phase systems

Author

Jinwook Lee

Keywords and Phrases

Liquid-liquid extraction

Abstract

"Human antibodies that are very important for the immune system can be produced in transgenic corn. However, their expression is low, less than 4% purity. The consequence of this low expression is an increase in the down-stream processing. Therefore, it is necessary to develop novel and economical purification processes to fully benefit from plant host systems. A recombinant human antibody expressed in corn was separated from its impurities using aqueous two-phase extraction. PEG/potassium phosphate, PEG/sodium citrate, and PEG/ammonium phosphate aqueous two-phase systems were used. The effect of pH, temperature, molecular weight of PEG, total concentration of the phase-forming species, and low concentration of NaCl were investigated to determine optimum separation conditions"--Abstract, page iii.

Advisor(s)

Forciniti, Daniel

Committee Member(s)

Ma, Yinfa
Reed, X. B., Jr.
Xing, Yangchuan
Ludlow, Douglas K.

Department(s)

Chemical and Biochemical Engineering

Degree Name

Ph. D. in Chemical Engineering

Sponsor(s)

Monsanto Protein Tech

Publisher

University of Missouri--Rolla

Publication Date

Spring 2006

Pagination

ix, 89 pages

Note about bibliography

Includes bibliographical references (pages 82-88).

Rights

© 2006 Jinwook Lee, All rights reserved.

Document Type

Dissertation - Citation

File Type

text

Language

English

Subject Headings

Extraction (Chemistry)Plant biotechnologyPolyethylene glycol -- BiotechnologyTransgenic plants

Thesis Number

T 8980

Print OCLC #

85836039

Recommended Citation

Lee, Jinwook, "Purification of human antibody from transgenic corn using aqueous two-phase systems" (2006). Doctoral Dissertations. 1663.
https://scholarsmine.mst.edu/doctoral_dissertations/1663