Automatic Cell Tracking Applied to Analysis of Cell Migration in Wound Healing Assay
The wound healing assay in vitro is widely used for research and discovery in biology and medicine. This assay allows for observing the healing process in vitro in which the cells on the edges of the artificial wound migrate toward the wound area. The influence of different culture conditions can be measured by observing the change in the size of the wound area. For further investigation, more detailed measurements of the cell behaviors are required. In this paper, we present an application of automatic cell tracking in phase-contrast microscopy images to wound healing assay. The cell behaviors under three different culture conditions have been analyzed. Our cell tracking system can track individual cells during the healing process and provide detailed spatio-temporal measurements of cell behaviors. The application demonstrates the effectiveness of automatic cell tracking for quantitative and detailed analysis of the cell behaviors in wound healing assay in vitro.
R. Bise et al., "Automatic Cell Tracking Applied to Analysis of Cell Migration in Wound Healing Assay," Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (2011, Boston, MA), pp. 6174-6179, Institute of Electrical and Electronics Engineers (IEEE), Aug 2011.
The definitive version is available at https://doi.org/10.1109/IEMBS.2011.6091525
Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (2011: Aug. 30-Sept. 3, Boston, MA)
Keywords and Phrases
Biology and Medicine; Cell Behaviors; Cell Migration; Cell Tracking; Cell Tracking System; Culture Conditions; Healing Process; In-Vitro; Individual Cells; Phase-Contrast Microscopy; Spatio-Temporal; Wound Healing; Cells; Cell Culture
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Article - Conference proceedings
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01 Aug 2011