Culture-Based Biochip for Environmental Monitoring
Abstract
In this paper, we describe the development of a culture-based biochip for detecting mycobacteria in environmental samples. The biochips use the paraffinophilic nature of mycobacteria to rapidly detect and differentiate them from non-target microorganisms. New methods of depositing and patterning paraffin were developed to fabricate prototype biochips. Biochips were experimentally tested to demonstrate rapid detection of mycobacteria in environmental samples collected from a municipal sewage treatment plant. Our successful demonstration of the culture-based biochip technology presents an alternative approach for developing new technology to track microorganisms in complex environmental samples.
Recommended Citation
G. Jing et al., "Culture-Based Biochip for Environmental Monitoring," Proceedings of SPIE - The International Society for Optical Engineering (2004, San Jose, CA), vol. 5345, pp. 68 - 77, SPIE, Jan 2004.
The definitive version is available at https://doi.org/10.1117/12.524610
Meeting Name
Microfluidics, BioMEMS, and Medical Microsystems II (2004: Jan. 26-27, San Jose, CA)
Department(s)
Civil, Architectural and Environmental Engineering
Sponsor(s)
SPIE
Keywords and Phrases
Biochips; Environmental monitoring; Myobacteria; Paraffin patterning; Adhesives; Bacteria; Biomass; Cell culture; Curing; Environmental engineering; Fluorescence; Health care; Potable water; RNA; Sorption; Spin coating; Microprocessor chips
International Standard Serial Number (ISSN)
0277-786X
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2004 SPIE, All rights reserved.
Publication Date
01 Jan 2004
