Removal of estrogenic activity of natural and synthetic hormones from a municipal wastewater: Efficiency of horseradish peroxidase and laccase from Trametes versicolor

Abstract

Some researches studied the removal of steroid estrogens by enzymatic treatment, however none verified the residual estrogenicity after the enzymatic treatment at environmental conditions. in this study, the residual estrogenic activities of the key natural and synthetic steroid estrogens were investigated following enzymatic treatment with horseradish peroxidase (HRP) and laccase from Trametes versicolor. Synthetic water and municipal wastewater containing environmental concentrations of estrone, 17β-estradiol, estriol, and 17α-ethinylestradiol were treated. Liquid chromatography-mass spectrometry analysis demonstrated that the studied steroid estrogens were completely oxidized in the wastewater reaction mixture after a 1-h treatment with either HRP (8-10 U ml-1) or laccase (20 U ml-1). using the recombinant yeast assay, it was also confirmed that both enzymatic treatments were very efficient in removing the estrogenic activity of the studied steroid estrogens. the laccase-catalyzed process seemed to present great advantages over the HRP-catalyzed system for up-scale applications for the treatment of municipal wastewater. © 2007 Elsevier Ltd. All rights reserved.

Department(s)

Civil, Architectural and Environmental Engineering

Comments

Ministerio de Ciencia y Tecnología, Grant BIO2005-00840

Keywords and Phrases

Endocrine disrupter; Estrogenicity; HRP; Laccase; Wastewater

International Standard Serial Number (ISSN)

0045-6535

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 Elsevier, All rights reserved.

Publication Date

01 Jan 2008

PubMed ID

17897698

Link to Full Text

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