Laser using -Based Indirect Fluorometric Detection and Quantification in Thin-Layer Chromatography

Author

Yinfa Ma, Missouri University of Science and TechnologyFollow
Lance B. Koutny
Edward S. Yeung

Abstract

A laser-based indirect fluorometric detection method for thin-layer chromatography is described. The new technique can be easily used for quantitative measurements because of its two-dimensional scanning capability. Since it is based on the indirect fluorescence mode, universal detection is possible without derivatization. Also, it takes only 35 s for acquiring a data array of 256 × 64 points with this technique to achieve a detection limit of 6 pg. This is 100 times lower than when the human eye is used as a detector for the same samples based on indirect fluorometry. The linearity in indirect fluorometric detection is found to be over 2 orders of magnitude.

Recommended Citation

Y. Ma et al., "Laser using -Based Indirect Fluorometric Detection and Quantification in Thin-Layer Chromatography," Analytical Chemistry, vol. 61, no. 17, pp. 1931 - 1933, American Chemical Society (ACS), Sep 1989.

The definitive version is available at https://doi.org/10.1021/ac00192a031

Department(s)

Chemistry

International Standard Serial Number (ISSN)

0003-2700

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1989 American Chemical Society (ACS), All rights reserved.

Publication Date

01 Sep 1989