Determination of Total Iron Binding Capacity of Serum by Capillary Electrophoresis

Abstract

A capillary electrophoresis (CE) technique for determining total iron binding capacity (TIBC) of serum has been developed. The optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with Fe+3; then, dissociate them completely after removing excess unbound Fe. Finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the CE analysis. Ammonium acetate (pH = 5.0) was used as CE background electrolyte solution. In this system, a good linear correlation coefficient was maintained over the range 0.5~10 µM (r = 0.9979, n=12). Seven adult serum samples were studied and the TIBC parameters measured. In the present system, 10~30 µL serum is sufficient for determination. The study shows that the CE technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application.

Department(s)

Chemistry

Keywords and Phrases

Iron; Accuracy; Capillary Electrophoresis; Chemical Analysis; Iron Blood Level; Metal Binding; Priority Journal; Serum Analysis; Total Iron Binding Capacity

International Standard Serial Number (ISSN)

0009-5893

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1999 Springer Verlag, All rights reserved.

Publication Date

01 Jan 1999

Link to Full Text

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