Pteridine Analysis in Urine by Capillary Electrophoresis using Laser- Induced Fluorescence Detection

Abstract

Pteridines are a class of compounds excreted in urine, the levels of which are found to elevate significantly in tumor-related diseases. For the first time, we have developed a method, based on high-performance capillary electrophoresis (HPCE) and laser-induced fluorescence (LIF) detection, to monitor the pteridine levels in urine. HPCE provides better separation than high-performance liquid chromatography and the LIF detector enables us to detect minute amounts of pteridines in body fluid. Eight different pteridine derivatives were well separated in 0.1 M Tris-0.1 M borate-2 mM EDTA buffer (pH 8.75) using a 60-cm fused-silica capillary (50-µm i.d., 35-cm effective length), six of which were detected and characterized in urine samples from normal persons and different cancer patients. The detection limits of these pteridines are under 1 x 10-10 M. The levels of neopterin, pterine, xanthopterin, and pterin-6-carboxylic acid were found to be significantly elevated in urine excreted by cancer patents, while the level of isoxanthopterin dropped in these patients. No significant change of biopterin level was found between healthy individuals and cancer patients. This method can be used in clinical laboratories either for cancer monitoring or for precancer screening.

Department(s)

Chemistry

Keywords and Phrases

Isoxanthopterin; Neopterin; Pteridine; Pterin Derivative; Xanthopterin; Analytic Method; Article; Capillary Electrophoresis; Chemical Analysis; Controlled Study; Fluorescence; Human; PH; Temperature Dependence; Urinalysis; Electrophoresis, Capillary; Fluorescence; Humans; Lasers; Pteridines

International Standard Serial Number (ISSN)

0003-2700

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1999 American Chemical Society (ACS), All rights reserved.

Publication Date

01 Apr 1999

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