Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A
Abstract
We have successfully developed gold nanoparticle based immunostrip assay to detect protein-A (PA). Rabbit polyclonal antibody IGg (αPA) that has affinity to PA was conjugated to gold nanoparticles (GNPs) and the gold nanoconjugate (αPA-GNP) was used to detect protein-A by simple immunostrip assay method. ELISA experiments were used to confirm the retention of binding affinity of antibody towards protein-A after conjugation with gold nanoparticles.
Recommended Citation
A. Zambre et al., "Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A," Applied Mechanics and Materials, vol. 229-231, pp. 260 - 266, Trans Tech Publications, Nov 2012.
The definitive version is available at https://doi.org/10.4028/www.scientific.net/AMM.229-231.260
Department(s)
Chemistry
Sponsor(s)
Science and Engineering Institute; Universiti Putra Malaysia
Keywords and Phrases
Assay methods; Binding affinities; ELISA; Gold nanoparticle; Gold Nanoparticles; Polyclonal antibody; Protein-A; Secondary antibodies; Antibodies; Binding energy; Metal nanoparticles; Gold; αPA; ELISA; Gold nanoparticles (GNP); Immunostrip assay; Protein-A (PA); Secondary antibody (GAR-IgG-HRP)
International Standard Book Number (ISBN)
978-3037855102
International Standard Serial Number (ISSN)
1660-9336
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2012 Trans Tech Publications, All rights reserved.
Publication Date
01 Nov 2012
