Superoxide Chemical Transformation of Diolepoxide Polyaromatic Hydrocarbon DNA Adducts. Determination of Benzo[α]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol by Gas Chromatography
Abstract
Benzo[α]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (100 pg, 342 fmol) was measured using the following sequence of steps: (1) chemical transformation with potassium superoxide to 2,3-pyrenedicarboxylic acid; (2) electrophore derivatization with pentafluorobenzyl bromide; (3) sample clean-up by high-performance liquid chromatography and (4) measurement by gas chromatography with electron-capture detection and by gas chromatography with electron-capture negative-ion mass spectrometry. The overall, absolute yields obtained by the two procedures were 69% and 60%, respectively. This work completes the first stage towards the establishment of a general method for detecting diolepoxide polyaromatic hydrocarbon DNA adducts by gas chromatography. © 1991.
Recommended Citation
W. Li et al., "Superoxide Chemical Transformation of Diolepoxide Polyaromatic Hydrocarbon DNA Adducts. Determination of Benzo[α]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol by Gas Chromatography," Journal of Chromatography A, Elsevier, Jan 1991.
The definitive version is available at https://doi.org/10.1016/0021-9673(91)85033-C
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 1991 Elsevier, All rights reserved.
Publication Date
01 Jan 1991
