Injection by Hydrostatic Pressure in Conjunction with Electrokinetic Force on a Microfluidic Chip

Abstract

A simple method was developed for injecting a sample on a cross-form microfluidic chip by means of hydrostatic pressure combined with electrokinetic forces. The hydrostatic pressure was generated simply by adjusting the liquid level in different reservoirs without any additional driven equipment such as a pump. Two dispensing strategies using a floating injection and a gated injection, coupled with hydrostatic pressure loading, were tested. The fluorescence observation verified the feasibility of hydrostatic pressure loading in the separation of a mixture of fluorescein sodium salt and fluorescein isothiocyanate. This method was proved to be effective in leading cells to a separation channel for single cell analysis.

Department(s)

Chemistry

Sponsor(s)

National Natural Science Foundation (China)

Keywords and Phrases

Fluorescein Isothiocyanate; Fluorescein Sodium; Analytic Method; Animal Cell; Cell Separation; Controlled Study; Device; Electric Potential; Equipment Design; Feasibility Study; Flow Kinetics; Fluid Flow; Fluorescence Analysis; Hydrostatic Pressure; Injection; Methodology; Microprocessor; Nonhuman; Pump; Separation Technique; Animals; Carps; Electromagnetic Fields; Electrophoresis, Microchip; Erythrocytes; Fluorescence; Hydrostatic Pressure; Microfluidics

International Standard Serial Number (ISSN)

0173-0835

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2004 John Wiley & Sons, All rights reserved.

Publication Date

01 Jun 2004

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