Abstract

Nicotinamide adenine dinucleotide (NAD+) cofactor regeneration is essential for enabling dehydrogenase-promoted biosynthesis for value-added chemicals. Heterogeneous catalytic cofactor regeneration, using supported metal catalysts, is an emerging approach and has shown great promise. However, mechanistic insight remains largely unexplored. In this work, a series of silica-supported platinum (Pt) catalysts have been prepared for NAD+ cofactor regeneration, to understand the roles of Pt particle size and structure. A turnover frequency (TOF) 'volcano plot' was obtained for Pt clusters in the range of 2.2–7.1 nm, with the maximum TOF (136 h−1) observed at 5.6 nm. Selective Pt site blockage with polyvinyl pyrrolidone (PVP) revealed that the significant structure sensitivity originated from the synergistic effect of under- and well-coordinated sites in size-varied Pt clusters. In addition, a facet preference was also identified, where the cofactor regeneration favored the Pt(100) surface more than Pt(111). These findings provide the first insight into NAD+ regeneration using heterogeneous Pt catalysts, which will be particularly useful for the rational design of supported metal catalysts.

Department(s)

Chemistry

Comments

Engineering and Physical Sciences Research Council, Grant EP/V048635/1

International Standard Serial Number (ISSN)

1463-9076

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2026 Royal Society of Chemistry, All rights reserved.

Publication Date

21 Jan 2026

PubMed ID

41502209

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Chemistry Commons

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