Abstract
Pentosan polysulfate, a semisynthetic polysaccharide, was employed as a chiral un buffer additive in capillary electrophoresis. Twenty-eight racemic analytes were resolved. The separations were successful only at low pH when the analytes were significantly protonated. This suggests that ionic interactions were the dominant associative interactions between the anionic pentosan polysulfate and the positively charged analytes. Compared to other linear, carbohydrate-based chiral selectors (i.e., chondroitin sulfates, heparin and dextran sulfate) pentosan polysulfate has some characteristics common of anionic polysaccharides; yet it has several differences in its structure and properties which account for its unusual enantioselectivity. The effects of pH, concentration of phosphate buffer, concentration of pentosan polysulfate and the type and concentration of organic modifier on the enantiomeric separations were investigated. The optimization of these separations were dependent on the nature of the analytes and could be achieved by the proper choice of experimental conditions.
Recommended Citation
X. Wang et al., "Separation of Enantiomers by Capillary Electrophoresis using Pentosan Polysulfate," Electrophoresis, vol. 20, no. 1, pp. 162 - 170, Wiley-VCH Verlag; Wiley, Jan 1999.
The definitive version is available at https://doi.org/10.1002/(SICI)1522-2683(19990101)20:1<162::AID-ELPS162>3.0.CO;2-6
Department(s)
Chemistry
Publication Status
Full Access
Keywords and Phrases
Anionic polysaccharides; Capillary electrophoresis; Chiral run buffer additive; Pentosan polysulfate; Separation of enantiomers
International Standard Serial Number (ISSN)
0173-0835
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Wiley-VCH Verlag; Wiley, All rights reserved.
Publication Date
01 Jan 1999
PubMed ID
10065973
Comments
National Institute of General Medical Sciences, Grant R01GM053825