High-performance Liquid Chromatographic and Capillary Electrophoretic Enantioseparation of Plant Growth Regulators and Related Indole Compounds using Macrocyclic Antibiotics as Chiral Selectors

Author

F. Hui
K. H. Ekborg-Ott
Daniel W. Armstrong, Missouri University of Science and Technology

Abstract

Enantioseparation of plant growth regulators, such as 3-(3-indolyl)-butyric acid, abscisic acid and structurally related molecules including a variety of substituted tryptophan compounds, has been achieved by HPLC and/or CE. The covalently bonded macrocyclic antibiotics, teicoplanin, ristocetin A and vancomycin, were used as chiral stationary phases (CSPs) in HPLC. Most of the racemates were baseline resolved in the reversed-phase mode (EtOH-H2O) using the teicoplanin CSP. The chiral recognition mechanism is discussed in regard to the structure of the analytes. In CE, the three aforementioned macrocyclic antibiotics were used as chiral additives in a phosphate run buffer. The effect of pH and the concentration of the organic modifiers were considered. The results obtained by HPLC and CE were compared. © 2001 Elsevier Science B.V.

Recommended Citation

F. Hui et al., "High-performance Liquid Chromatographic and Capillary Electrophoretic Enantioseparation of Plant Growth Regulators and Related Indole Compounds using Macrocyclic Antibiotics as Chiral Selectors," Journal of Chromatography A, vol. 906, no. 1 thru 2, pp. 91 - 103, Elsevier, Jan 2001.

The definitive version is available at https://doi.org/10.1016/S0021-9673(00)00954-7

Department(s)

Chemistry

Comments

National Institutes of Health, Grant R01GM053825

Keywords and Phrases

Enantiomer separation; Indole compounds; Plant growth regulators; Ristocetin A; Teicoplanin; Vancomycin

International Standard Serial Number (ISSN)

0021-9673

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 Elsevier, All rights reserved.

Publication Date

12 Jan 2001

PubMed ID

11215905