A Comparative Study of Buckminsterfullerene and Higher Fullerene Separations by HPLC
Abstract
Several HPLC columns that have been recommended for the separation of fullerenes were compared. Each column (i.e., stationary phase) had a different optimum mobile phase. In general, mobile phases consisted of binary mixtures of a "good" fullerene solvent and a "poorer" fullerene solvent. Reversed phase stationary phases with alkyl (aliphatic) substituents produced superior analytical separations for all fullerenes. However, stationary phases with aromatic substituents were better for preparative separations. Increasing the proportion of the "poor" fullerene solvent in the mobile phase generally increased resolution and retention but decreased the mass load. Injecting too high a concentration of fullerenes in any column caused peak splitting to occur. The fullerene retention order was the same on all columns and with all mobile phases. The fullerene separation ability of all columns tended to deteriorate with time. Overloading the column or doing preparative separations greatly accelerated the deterioration process. It is believed that irreversible adsorption of fullerenes or fullerene by-products is responsible for the degradation of column performance. © 1995, Taylor & Francis Group, LLC. All rights reserved.
Recommended Citation
M. P. Gasper and D. W. Armstrong, "A Comparative Study of Buckminsterfullerene and Higher Fullerene Separations by HPLC," Journal of Liquid Chromatography, vol. 18, no. 6, pp. 1047 - 1076, Taylor and Francis Group; Taylor and Francis, Mar 1995.
The definitive version is available at https://doi.org/10.1080/10826079508009276
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0148-3919
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Taylor and Francis Group; Taylor and Francis, All rights reserved.
Publication Date
01 Mar 1995
Comments
U.S. Department of Energy, Grant DE FG02 88ER13819