Ce Resolution of Neutral and Anionic Racemates with Glycopeptide Antibiotics and Micelles
Abstract
The glycopeptide antibiotics vancomycin, ristocetin A and teicoplanin have been shown to be effective chiral selectors in capillary electrophoresis (CE). However, most of the resolved enantiomers were anionic or acidic compounds that contained carboxylic acid, phosphate, or sulfonate groups. Conversely, a large number of neutral racemates are resolved using these same chiral selectors attached to the stationary phase in HPLC. The problem in resolving neutral analytes in CE is outlined and discussed. Addition of sodium dodecyl sulfate (SDS) micelles to the run buffer greatly increases the available elution window, thereby facilitating the separation of neutral racemates. Neutral analytes resolved in this CE system include bromacil, bendroflumethiazide and 5-(4-hydroxypheny1)-5- phenylhydantion. The separations of coumachlor and warfarin are also demonstrated. In addition, the effect of surfactant concentration and organic co-solvents on elution time and enantioresolution are discussed. © 1995, Taylor & Francis Group, LLC. All rights reserved.
Recommended Citation
D. W. Armstrong and K. L. Rundlett, "Ce Resolution of Neutral and Anionic Racemates with Glycopeptide Antibiotics and Micelles," Journal of Liquid Chromatography, vol. 18, no. 18 thru 19, pp. 3659 - 3674, Taylor and Francis Group; Taylor and Francis, Nov 1995.
The definitive version is available at https://doi.org/10.1080/10826079508014617
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0148-3919
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Taylor and Francis Group; Taylor and Francis, All rights reserved.
Publication Date
01 Nov 1995
Comments
U.S. Department of Energy, Grant DE FG02 88ER13819