Methods for the Estimation of Binding Constants by Capillary Electrophoresis

Author

Kimber L. Rundlett
Daniel W. Armstrong, Missouri University of Science and Technology

Abstract

Capillary electrophoresis (CE) has developed into a particularly effective means to determine apparent equilibrium constants for molecular association in solution (e.g., to micelles, cyclodextrins, antibiotics, proteins, RNA, DNA, etc.). The various experimental, graphical and mathematical approaches for determining association constants are reviewed. In CE, association constants can be calculated because there is a relationship between substrate concentration and the measured electrophoretic mobility of the solute. Most of the approaches for obtaining association constants by CE are conceptually and mathematically related to one another. Likewise, they are analogous to many spectroscopic techniques that are used for obtaining association constants. The advantages, limitations and proper use of the various CE approaches are examined.

Recommended Citation

K. L. Rundlett and D. W. Armstrong, "Methods for the Estimation of Binding Constants by Capillary Electrophoresis," Electrophoresis, vol. 18, no. 12 thru 13, pp. 2194 - 2202, Wiley-VCH Verlag; Wiley, Jan 1997.

The definitive version is available at https://doi.org/10.1002/elps.1150181210

Department(s)

Chemistry

Keywords and Phrases

Equilibrium constants; Errors; Linear plotting; Molecular association; Nonlinear regression

International Standard Serial Number (ISSN)

0173-0835

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 Wiley-VCH Verlag; Wiley, All rights reserved.

Publication Date

01 Jan 1997

PubMed ID

9456034