Determination of Association Constants by Chromatography and Electrophoresis

Abstract

In order to understand any solution-based dynamic chemical system, it is necessary to have basic knowledge of the equilibrium conditions and the association behavior of the components. It is apparent that the separation techniques of chromatography and electrophoresis are useful for determining association constants and for studying intermolecular interactions in a wide variety of chemical and biochemical systems. When properly used, they produce accurate data in an efficient manner. One's choice of technique (LC, HPLC, TLC, GPC, electrophoresis, or CE) or of data treatment (various linear plotting methods, nonlinear regression, mobility ratio, etc.) can vary with the nature of the system to be studied, the accuracy needed, and the experimental constraints inherent in every project. Frequently, the separation-based approaches are the best way and sometimes the only effective way to evaluate association behavior in dynamic solution systems.

Department(s)

Chemistry

International Standard Serial Number (ISSN)

0065-2415

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 The Authors, All rights reserved.

Publication Date

01 Dec 1998

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