Abstract
Detectable levels of four free D-amino acids (phenylalanine, tyrosine, tryptophan and leucine) were found in human urine. This was accomplished using a liquid chromatographic coupled column (i.e., achiral-chiral) method that was developed for the rapid and sensitive analysis of these compounds. The technique was tested on a series of commercial L-amino acids. Care was taken to minimize racemization. Trace to percent levels of contaminating D-enantiomers were found in all commercial samples. In urine the D-amino acids ranged from several hundredths of a percent up to percent levels of the corresponding excreted natural L-amino acids. There were no apparent correlations between the amount of amino acids excreted and the relative amount of D-enantiomers present. Currently this study is being expanded to include additional amino acids and a variety of other physiological fluids. © 1991 Springer-Verlag.
Recommended Citation
D. W. Armstrong et al., "Evaluation Of D-amino Acid Levels In Human Urine And In Commercial L-amino Acid Samples," Amino Acids, vol. 1, no. 1, pp. 97 - 106, Springer, Feb 1991.
The definitive version is available at https://doi.org/10.1007/BF00808096
Department(s)
Chemistry
Second Department
Mechanical and Aerospace Engineering
Keywords and Phrases
D-amino acids; Separation of racemic amino acids
International Standard Serial Number (ISSN)
1438-2199; 0939-4451
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2023 Springer, All rights reserved.
Publication Date
01 Feb 1991
