Abstract
A total of 121 racemic compounds were separated In the normal-phase mode on a (S)-(1-naphthylethyl)carbamoylated β-cyclodextrin (S-NEC-β-CD) bonded phase and 74 on the R equivalent (-NEC) chiral stationary phase (CSP). All compounds are of the type that have four substituents on a stereogenlc center, rather than an "axis of chirality". It is shown that the binary solvent pair used as the mobile phase has a significant Influence on chiral recognition. However, the proportions of the components of a specific pair have little effect. From the results, the Individual contributions to chiral recognition by these CSPs were estimated for 81 different substituents of the stereogenlc center. Varying the arrangement of these 81 substituents could produce over 1.8 million compounds. Hydrogen was chosen as the reference substituent and was assigned a 0 cal/mol free energy. The chiral recognition Increased when sp2-hybrldlzed carbons were connected to the stereogenlc center. Conversely, sp3-hybrldlzed carbons decreased the enantioselectivity. Amldo groups Increased the chiral recognition, especially when associated with π-acid (3, 5-dlnltrobenzoyl) or π-basic (naphthyl) groups. This approach does not allow one to know which enantiomer elutes first. However, the "substituent energy" list for chiral compounds can be used to obtain an estimated value for the enantioselectivity of a compound by adding the energy contributions of the four substituents connected to the stereogenlc center. In this way one can predict a priori whether or not a compound will separate on a CSP and estimate Its separation factor (α). Theoretically, this approach can be used for most CSPs, provided a sufficient data base Is generated on them. © 1992, American Chemical Society. All rights reserved.
Recommended Citation
A. Berthod et al., "Empirical Procedure That Uses Molecular Structure To Predict Enantioselectivity Of Chiral Stationary Phases," Analytical Chemistry, vol. 64, no. 4, pp. 395 - 404, American Chemical Society, Jan 1992.
The definitive version is available at https://doi.org/10.1021/ac00028a014
Department(s)
Chemistry
International Standard Serial Number (ISSN)
1520-6882; 0003-2700
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2023 American Chemical Society, All rights reserved.
Publication Date
01 Jan 1992
PubMed ID
1616129
