Enantiomeric Separation Of Fluorescent, 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate, Tagged Amino Acids

Author

Maria Pawlowska
Shushi Chen
Daniel W. Armstrong, Missouri University of Science and Technology

Abstract

A new derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), has been used successfully for chromatographic enantioseparation of 31 amino acids on cyclodextrin bonded stationary phases. AQC reacts with both primary and secondary amino acids to produce stable and highly fluorescent derivatives suitable for efficient and sensitive chromatographic determinations. The derivatization reaction proceeds without detectable racemization. The detection limit is in the femtomole range and approximately 0.0075% of the d-enantiomer in an excess of the l-enantiomer is detectable. High resolution values are needed when determining trace enantiomeric impurities. © 1993.

Recommended Citation

M. Pawlowska et al., "Enantiomeric Separation Of Fluorescent, 6-aminoquinolyl-N-hydroxysuccinimidyl Carbamate, Tagged Amino Acids," Journal of Chromatography A, vol. 641, no. 2, pp. 257 - 265, Elsevier, Jul 1993.

The definitive version is available at https://doi.org/10.1016/0021-9673(93)80142-U

Department(s)

Chemistry

Comments

National Institute of General Medical Sciences, Grant NIH BMT 2ROl GM36292-05A2

International Standard Serial Number (ISSN)

0021-9673

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2023 Elsevier, All rights reserved.

Publication Date

09 Jul 1993

PubMed ID

8366160