Structure Of An Alkali-soluble Polysaccharide From The Hyphal Wall Of The Basidiomycete Coprinus Macrorhizus Var. Microsporus
Abstract
A unique, alkali-soluble polysaccharide has been isolated from the cell walls of the basidiomycete Coprinus macrorhizus microsporus. The polysaccharide, which is primarily a glucan, contains a large proportion of α-(1→4)-linked d-glucose residues and a smaller amount of β-(1→3) and (1→6) linkages, as suggested by methylation, partial acid hydrolysis, periodate oxidation, and enzymic studies. Hydrolysis of the methylated polysaccharide gave equimolar amounts of 2,4-di- and 2,3-di-O-methyl-d-glucose; no 2,6-di-O-methyl-d-glucose was identified, indicating the absence of branch points joined through O-1, O-3, and O-4. The isolation and identification of 2-O-α- glucopyranosylerythritol from the periodate-oxidized polysaccharide suggests that segments of the a-(1→4)-linked d-glucose residues are joined by single (1→3)-linkages. An extracellular enzyme-preparation from Sporotrichum dimorphosporum (QM 806) containing both β-(1→3)- and α-(1→4)-d-glucanohydrolase activity released 76% of the reducing groups from the polysaccharide. The polysaccharide also contains minor proportions of xylose, mannose, 2-amino-2-deoxyglucose, and amino acids. © 1979.
Recommended Citation
C. B. Bottom and D. J. Siehr, "Structure Of An Alkali-soluble Polysaccharide From The Hyphal Wall Of The Basidiomycete Coprinus Macrorhizus Var. Microsporus," Carbohydrate Research, vol. 77, no. 1, pp. 169 - 181, Elsevier, Jan 1979.
The definitive version is available at https://doi.org/10.1016/S0008-6215(00)83803-3
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0008-6215
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2023 Elsevier, All rights reserved.
Publication Date
01 Jan 1979
