Glutamine Protects Chinese Hamster Ovary Cells From Radiation Killing
Abstract
Chinese Hamster Ovary (CHO) cells were propagated in vitro and exposed to varying doses of ionizing radiation. The surviving fraction of cells was determined, being found to be a function of the radiation dose. The cell survival curves obtained as a function of radiation dose were modified by the inclusion of varying doses of glutamine in the medium with glutamine demonstrating a radioprotective effect. The radioprotectant effect of glutamine for CHO cells was more pronounced at higher radiation doses. Glutamine has been categorized as a non-essential amino acid in that it can be synthesized in some tissues; however, a number of cell lines require glutamine to survive and grow in vitro and supplementation of glutamine has been found to ameliorate the stress of surgery or irradiation to the gastrointestinal tract. It may be appropriate to consider glutamine as a conditionally essential amino acid. © 1994.
Recommended Citation
R. Winters et al., "Glutamine Protects Chinese Hamster Ovary Cells From Radiation Killing," Life Sciences, vol. 55, no. 9, pp. 713 - 720, Elsevier, Jan 1994.
The definitive version is available at https://doi.org/10.1016/0024-3205(94)00679-2
Department(s)
Chemistry
Keywords and Phrases
glutamine; ovary; radiation
International Standard Serial Number (ISSN)
0024-3205
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2023 Elsevier, All rights reserved.
Publication Date
01 Jan 1994
PubMed ID
8065234
