Covalent Linkage of One-Dimensional DNA Arrays Bonded by Paranemic Cohesion
Abstract
The construction of DNA nanostructures from branched DNA motifs, or tiles, typically relies on the use of sticky-ended cohesion, owing to the specificity and programmability of DNA sequences. The stability of such constructs when unligated is restricted to a specific range of temperatures, owing to the disruption of base pairing at elevated temperatures. Paranemic (PX) cohesion was developed as an alternative to sticky ends for the cohesion of large topologically closed species that could be purified reliably on denaturing gels. However, PX cohesion is also of limited stability. In this work, we added sticky-ended interactions to PX-cohesive complexes to create interlocked complexes by functionalizing the sticky ends with psoralen, which can form cross-links between the two strands of a double helix. We were able to reinforce the stability of the constructs by creating covalent linkages between the 3′-ends and 5′-ends of the sticky ends; the sticky ends were added to double crossover domains via 3′-3′ and 5′-5′ linkages. Catenated arrays were obtained either by enzymatic ligation or by UV cross-linking. We have constructed finite-length one-dimensional arrays linked by interlocking loops and have positioned streptavidin-gold particles on these constructs.
Recommended Citation
Y. P. Ohayon et al., "Covalent Linkage of One-Dimensional DNA Arrays Bonded by Paranemic Cohesion," ACS Nano, vol. 9, no. 10, pp. 10304 - 10312, American Chemical Society (ACS), Sep 2015.
The definitive version is available at https://doi.org/10.1021/acsnano.5b04335
Department(s)
Chemistry
Keywords and Phrases
3',3' and 5',5' linkages; catenanes; ligated DNA constructs; one-dimensional DNA arrays; paranemic cohesion; topological linkage; UV cross-linking
International Standard Serial Number (ISSN)
1936-0851; 1936-086X
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2015 American Chemical Society (ACS), All rights reserved.
Publication Date
01 Sep 2015
PubMed ID
26343906