Separating Microbes in the Manner of Molecules. 1. Capillary Electrokinetic Approaches

Abstract

Selective, high-efficiency separations of intact bacteria may, in some cases, allow them to be identified and quantified in much the same way that molecules are done today. Two different capillary electrokinetic approaches were utilized. The first approach used a dissolved polymer-based CE separation that may be affected by size and shape considerations. Another approach uses capillary isoelectric focusing to separate bacteria by their surface charge or isoelectric point. Good peak shapes and extremely high efficiencies are observed (up to ~ 1 600 000 theoretical plates/m). Careful sample preparation and separation runs are essential in order to obtain reproducible separations. Expansion of these types of rapid, efficient microbial separations could have profound effects on many branches of science and technology.

Department(s)

Chemistry

Second Department

Biological Sciences

Keywords and Phrases

Bacterium Identification; Capillary Electrophoresis; Enterobacter aerogenes; Escherichia coli; Isoelectric Point; Micrococcus luteus; Pseudomonas putida; Reproducibility; Saccharomyces cerevisiae; Serratia; Surface Charge; Electrophoresis, Capillary; Isoelectric Focusing; Microbiological Techniques

International Standard Serial Number (ISSN)

0003-2700; 1520-6882

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1999 American Chemical Society (ACS), All rights reserved.

Publication Date

01 Dec 1999

PubMed ID

10624154

Link to Full Text

Share

 
COinS