Separating Microbes in the Manner of Molecules. 1. Capillary Electrokinetic Approaches
Selective, high-efficiency separations of intact bacteria may, in some cases, allow them to be identified and quantified in much the same way that molecules are done today. Two different capillary electrokinetic approaches were utilized. The first approach used a dissolved polymer-based CE separation that may be affected by size and shape considerations. Another approach uses capillary isoelectric focusing to separate bacteria by their surface charge or isoelectric point. Good peak shapes and extremely high efficiencies are observed (up to ~ 1 600 000 theoretical plates/m). Careful sample preparation and separation runs are essential in order to obtain reproducible separations. Expansion of these types of rapid, efficient microbial separations could have profound effects on many branches of science and technology.
D. W. Armstrong et al., "Separating Microbes in the Manner of Molecules. 1. Capillary Electrokinetic Approaches," Analytical Chemistry, vol. 71, no. 24, pp. 5465-5469, American Chemical Society (ACS), Dec 1999.
The definitive version is available at https://doi.org/10.1021/ac990779z
Keywords and Phrases
Bacterium Identification; Capillary Electrophoresis; Enterobacter aerogenes; Escherichia coli; Isoelectric Point; Micrococcus luteus; Pseudomonas putida; Reproducibility; Saccharomyces cerevisiae; Serratia; Surface Charge; Electrophoresis, Capillary; Isoelectric Focusing; Microbiological Techniques
International Standard Serial Number (ISSN)
Article - Journal
© 1999 American Chemical Society (ACS), All rights reserved.
01 Dec 1999